【英文标准名称】:StandardTestMethodforAdenosineTriphosphate(ATP)ContentofMicroorganismsinWater
【原文标准名称】:水中微生物的三磷酸腺甙含量的标准试验方法
【标准号】:ASTMD4012-1981(2009)
【标准状态】:现行
【国别】:美国
【发布日期】:1981
【实施或试行日期】:
【发布单位】:美国材料与试验协会(US-ASTM)
【起草单位】:D19.24
【标准类型】:(TestMethod)
【标准水平】:()
【中文主题词】:
【英文主题词】:Adenosinetriphosphate(ATP)content;ATPfireflymethod;Biomass;Filters/filterprocedures--watertesting;Microorganisms;Opticalmaterials/properties/tests--water;Organiccompounds--water;Pathogens--water;Photoluminescence;Sludge;Toxicity/toxicol
【摘要】:Arapidandroutineprocedurefordeterminingbiomassofthelivingmicroorganismsincultures,waters,wastewaters,andinplanktonandperiphytonsamplestakenfromsurfacewatersisfrequentlyofvitalimportance.However,classicaltechniquessuchasdirectmicroscopecounts,turbidity,organicchemicalanalyses,celltagging,andplatecountsareexpensive,time-consuming,ortendtounderestimatetotalnumbers.Inaddition,someofthesemethodsdonotdistinguishbetweenlivingandnonlivingcells.TheATPfirefly(luciferin-luciferase)methodisarapid,sensitivedeterminationofviablemicrobialbiomass.ATPistheprimaryenergydonorforlifeprocesses,doesnotexistinassociationwithnonlivingdetritalmaterial,andtheamountofATPperunitofbiomass(expressedinweight)isrelativelyconstant.(ATPpercellvarieswithspeciesandphysiologicalstateoftheorganism.)Thistestmethodcanbeusedto:Estimateviablemicrobialbiomassincultures,waters,andwastewaters.Estimatetheamountoftotalviablebiomassinplanktonandperiphytonsamples.EstimatethenumberofviablecellsinaunispeciescultureiftheATPcontent(oriftheaverageamountofATP)percellisknown.Estimateanddifferentiatebetweenzooplanktonic,phytoplanktonic,bacterial,andfungalATPthroughsizefractionationofwater,andwastewatersamples.Measurethemortalityrateofmicroorganismsintoxicitytestsinentrainmentstudies,andinothersituationswherepopulationsorassemblagesofmicroorganismsareplacedunderstress.1.1Thistestmethodcoversthemeasurementofadenosinetriphosphate(ATP)inmicroorganismsinconcentrationsnormallyfoundinlaboratorycultures,waters,wastewaters,andinplanktonandperiphytonsamplesfromwaters.1.2KnowledgeoftheconcentrationofATPcanberelatedtoviablebiomassormetabolicactivity,orbyutilizinganaverageconcentration(oramount)ofATPpercell,anestimatedcountofmicroorganismscanbeobtainedinthecaseofunispeciescultures.1.3Thistestmethodoffersahighdegreeofsensitivity,rapidity,accuracy,andreproducibility.However,extremecaremustbetakenateachstepintheanalysistoensuremeaningfulandreliableresults.1.4Theanalystshouldbeawarethattheprecisionstatementpertainsonlytodeterminationsinreagentwaterandnotnecessarilyinthematrixbeingtested.1.5ThevaluesstatedinSIunitsaretoberegardedasstandard.Nootherunitsofmeasurementareincludedinthisstandard.1.6Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappropriatesafetyandhealthpracticesanddeterminetheapplicabilityofregulatorylimitationspriortouse.
【中国标准分类号】:Z16
【国际标准分类号】:13_060_50
【页数】:4P.;A4
【正文语种】:英语